Validity and Reliability of a Novel Salivary Immunoassay for Individual Profiling in Applied Sports Science
DOI: 10.1080/15438627.2015.1005300
Title: Validity and Reliability of a Novel Salivary Immunoassay for Individual Profiling in Applied Sports Science
Journal Title: Research in Sports Medicine: An International Journal
Volume: pages 1-11
Issue: pages 1-11
Publication Date: pages 1-11
Start Page: pages1-11
End Page: pages1-11
ISSN: 1543-8627
Author: Sam Coada*, Christopher Mclellana, Timothy Whitehousea & Bon Graya
Affiliations:
a Faculty of Health Sciences and Medicine, Bond University, Queensland, Australia
Abstract: The aim of this study was to examine the validity and reliability of a novel immunoassay, developed to assess salivary Immunoglobulin A (s-IgA). Validity and reliability of the Individual Profiling Lateral Flow Device (IPRO LFD) for s-IgA concentrations ([s-IgA]) was assessed in males (n = 12) and females (n =13) who were involved in recreational activities. Reliability of the IPRO LFD method was assessed by comparing [s-IgA] of two saliva samples collected concurrently, while validity was assessed by comparing with an enzyme-linked immunosorbent assay (ELISA) method. The IPRO LFD had a strong positive correlation (r = 0.93, p < 0.001), with no difference in [s-IgA] compared with the ELISA. The IPRO LFD was considered reliable (ICC r = 0.89, p < 0.001 and CV = 9.40 %) for measures of [s-IgA]. We concluded that the IPRO LFD method may be a substitute to the ELISA method for measurements of [s-IgA].
Accepted: 20 Nov 2014

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