Autoregulation of thromboinflammation on biomaterial surfaces by a multicomponent therapeutic coating
DOI: 10.1016/j.biomaterials.2012.10.040
Title: Autoregulation of thromboinflammation on biomaterial surfaces by a multicomponent therapeutic coating
Journal Title: Biomaterials
Volume: 34
Issue: 4
Publication Date: January 2013
Start Page: 985
End Page: 994
Published online: online 5 November 2012
ISSN: 0142-9612
Affiliations:

  • a School of Natural Sciences, Linnaeus University, Sweden

  • b Department of Immunology, Genetics and Pathology, The Rudbeck Laboratory C5, Uppsala University, Dag Hammarskölds väg 20, Uppsala SE-751 85, Sweden

  • c Department of Pathology and Laboratory Medicine, University of Pennsylvania, USA

  • d Institute for Frontier Medical Sciences, Kyoto University, Japan
  • Abstract: on of the thrombotic and complement systems is the main recognition and effector mechanisms in the multiple adverse biological responses triggered when biomaterials or therapeutic cells come into blood contact. We have created a surface which is auto-protective to human innate immunity by combining three fundamentally different strategies, all developed by us previously, which have been shown to induce substantial, but incomplete hemocompatibility when used separately. In summary, we have conjugated a factor H–binding peptide; and an ADP-degrading enzyme; using a PEG linker on both material and cellular surfaces. When exposed to human whole blood, factor H was specifically recruited to the modified surfaces and inhibited complement attack. In addition, activation of platelets and coagulation was efficiently attenuated, by degrading ADP. Thus, by inhibiting thromboinflammation using a multicomponent approach, we have created a hybrid surface with the potential to greatly reduce incompatibility reactions involving biomaterials and transplantation.
    Accepted: 12 October 2012
    Received: 30 July 2012
    Keywords: Surface modification; poly(ethylene glycol) (PEG); Factor H-binding peptide; Apyrase; Complement; Coagulation
    Email: bo.nilsson@igp.uu.se

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