The osteogenic activity of strontium loaded titania nanotube arrays on titanium substrates
DOI: 10.1016/j.biomaterials.2012.09.041
Title: The osteogenic activity of strontium loaded titania nanotube arrays on titanium substrates
Journal Title: Biomaterials
Volume: 34
Issue: 1
Publication Date: January 2013
Start Page: 19
End Page: 29
Published online: online 6 October 2012
ISSN: 0142-9612
Affiliations:

  • a Department of Periodontology and Oral Medicine, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China

  • b School of Materials and Metallurgy, Wuhan University of Science and Technology, Wuhan 430081, China

  • c Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China

  • d State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, CAS, Shanghai, 200050, China

  • e Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China
  • Abstract: ent of biomedical titanium implants with high osteogenic ability for fast and good osseointegration under normal as well as osteoporotic conditions is hotly pursued. Strontium (Sr) loaded nanotubular structures (NT-Sr) that allow controlled and long-term Sr release are expected to yield favorable osteogenic effects and properties. NT-Sr structures with different tube diameters are fabricated by hydrothermal treatment of titania nanotubes formed at 10 and 40V (NT10 and NT40). The loaded Sr amounts are regulated by the hydrothermal treatment time of 1 and 3h (samples NT10-Sr1, NT10-Sr3, NT40-Sr1 and NT40-Sr3) in the Sr(OH)2 solution. Long lasting and controllable Sr release is observed from the NT-Sr samples with no cytotoxicity. The samples NT10 and NT10-Sr have multiple nanocues, comprising bundles of nanotubes of less than or equal to 30nm with bundle diameters between 100 and 400nm separated by about 80nm. Sr incorporation enhances proliferation of rat mesenchymal stem cells (MSCs) on the NT structure, especially NT10-Sr which promotes the spread of the MSCs into a polygonal osteoblastic shape. Both the NT and NT-Sr samples promote osteogenesis to varying degrees as indicated by gene expression and among the various samples, samples NT10-Sr3 and NT40-Sr significantly up-regulate the expressions of the osteogenesis related genes in the absence of an extra osteogenic agent. Samples NT10 and NT10-Sr generate big nodular alkaline phosphatase (ALP) products and induce extracellular matrix (ECM) mineralization, and the effects on NT10-Sr3 are most obvious due to the multiple scaled nanostructure and proper amount of incorporated Sr. In comparison, less ALP products and failure to induce ECM mineralization are observed from sample NT40-Sr, possibly due to cell function impairment by the uneven protein distribution. NT10-Sr3 which shows excellent osteogenic properties is very attractive and has large clinical potential.
    Accepted: 20 September 2012
    Received: 31 July 2012
    Tel: +852 27887724
    Email: zhaolingzhou1983@hotmail.com kaifuhuo@cityu.edu.hk kfhuo@wust.edu.cn paul.chu@cityu.edu.hk

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