Engineering extracellular matrix structure in 3D multiphase tissues
DOI: 10.1016/j.biomaterials.2011.05.043
Title: Engineering extracellular matrix structure in 3D multiphase tissues
Journal Title: Biomaterials
Volume: 32
Issue: 32
Publication Date: November 2011
Start Page: 8067
End Page: 8076
Published online: online 12 August 2011
ISSN: 0142-9612
Author: Brian M. Gillette, Ninna S. Rossen, Nikkan Das, Debra Leong, Meixin Wang, Arushi Dugar, Samuel K. Sia
Affiliations:
  • Department of Biomedical Engineering Columbia University, 351 Engineering Terrace, 500 W 120th Street, New York, NY 10027, USA
  • Abstract: e tissues, microscale variations in the extracellular matrix (ECM) structure can drive different cellular behaviors. Although control over ECM structure could prove useful in tissue Engineering and in studies of cellular behavior, isotropic 3D matrices poorly replicate variations in local microenvironments. In this paper, we demonstrate a method to engineer local variations in the density and size of collagen fibers throughout 3D tissues. The results showed that, in engineered multiphase tissues, the structures of collagen fibers in both the bulk ECM phases (as measured by mesh size and width of fibers) as well as at tissue interfaces (as measured by density of fibers and thickness of tissue interfaces) could be modulated by varying the collagen concentrations and gelling temperatures. As the method makes use of a previously published technique for tissue bonding, we also confirmed that significant adhesion strength at tissue interfaces was achieved under all conditions tested. Hence, this study demonstrates how collagen fiber structures can be engineered within all regions of a multiphase tissue scaffold by exploiting knowledge of collagen assembly, and presents an approach to engineer local collagen structure that complements methods such as flow alignment and electrospinning.
    Accepted: 12 May 2011
    Received: 29 April 2011
    Email: ss2735@columbia.edu

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