A combined assay of three lysosomal marker enzymes: Acid phosphatase, β-d-glucuronidase, and β-N-acetyl-d-hexosaminidase
DOI: 10.1016/0003-2697(84)90470-6
Title: A combined assay of three lysosomal marker enzymes: Acid phosphatase, β-d-glucuronidase, and β-N-acetyl-d-hexosaminidase
Journal Title: Analytical Biochemistry
Volume: 142
Issue: 2
Publication Date: 1 November 1984
Start Page: 312
End Page: 316
Published online: online 6 December 2004
ISSN: 0003-2697
Author: Bernd-Michael Lö;ffler, Bernd Hesse, Hans Kunze
Affiliations:
  • Department of Biochemical Pharmacology, Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Strasse 3, 3400 Göttingen, Federal Republic of Germany
  • Abstract: fied and rapid method for simultaneous activity measurements of three lysosomal marker enzymes, acid phosphatase, β-glucuronidase, and β-N-acetyl-d-hexosaminidase is described. The incubation is carried out in a single test tube and stopped by adding an alkaline sodium dodecyl sulfate solution, thus avoiding centrifugations and allowing for higher Triton X-100 concentrations in the incubation media. Two products of the β-glycosidases (phenolphthalein and 2-nitrophenolate) are measured spectrophotometrically at the respective wavelengths (555 and 420 nm), and one of the acid phosphatase products is quantitatively determined by measuring inorganic phosphate.
    Received: 3 April 1984
    Keywords: marker enzymes; acid phosphatase; β-glucuronidase; β-N-acetyl-d-hexosaminidase; lysosomes; enzyme assays

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