Ribosomal proteins. VII: Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins
DOI: 10.1016/0003-2697(70)90376-3
Title: Ribosomal proteins. VII: Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins
Journal Title: Analytical Biochemistry
Volume: 36
Issue: 2
Publication Date: August 1970
Start Page: 401
End Page: 412
Published online: online 7 December 2004
ISSN: 0003-2697
Affiliations:
  • Max-Planck-Institut für Molekulare Genetik, Berlin-Dahlem, Germany
  • Abstract: g>Two-dimensional polyacrylamide gel system has been developed, improving the analytical separation of complex protein mixtures as obtained from ribosomes. An equipment is described in detail (Figs. 1–4) which permits a simultaneous electrophoretic separation of five protein mixtures under identical conditions.By this method the protein mixtures of E. coli ribosomes can be resolved into about 50 components (Fig. 5). The use of this fingerprinting technique facilitates and accelerates considerably a number of investigations on the structure of ribosomes.
    Received: 7 January 1970

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