Improving HPLC separation performance using parallel segmented flow chromatography
DOI: 10.1016/j.microc.2012.11.001
Title: Improving HPLC separation performance using parallel segmented flow chromatography
Journal Title: Microchemical Journal
Volume: 111
Publication Date: July 2013
Start Page: 3
End Page: 7
Published online: online 13 November 2012
ISSN: 0026-265X

  • a Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW, Australia

  • b Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK
  • Abstract: HPLC columns are heterogeneous in their packing density, both in the axial and radial directions. Radial heterogeneity has a very serious deleterious effect on separation performance, which ultimately results in band distortions resulting in parabolic-like band profiles. Frictional heating and imperfect frit/distributor behaviour adds to the nature of band distortion. Recently, a new column technology has been developed, known as parallel segmented flow chromatography, which overcomes many of the limitations associated with band distortions. These columns separate mobile phase that elutes from the central section of the column, from mobile phase that elutes near the wall section. Improvements in plate numbers are substantial, with gains of 40% not uncommon, with a gain in sensitivity of between 45 and 80% (LOD) relative to conventional columns. We will discuss the principles of parallel segmented flow chromatography in addition to the separation performance under isocratic and gradient elution conditions, linearity, limit of detection and limit of quantification.
    Accepted: 3 November 2012
    Received: 3 August 2012
    Revised: 23 October 2012
    Fax: +?61 296859915

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