Structural characterization of red wine rhamnogalacturonan II
DOI: 10.1016/0008-6215(96)00139-5
Title: Structural characterization of red wine rhamnogalacturonan II
Journal Title: Carbohydrate Research
Volume: 290
Issue: 2
Publication Date: 2 September 1996
Start Page: 183
End Page: 197
Published online: online 12 December 1998
ISSN: 0008-6215
Author: Patrice Pellerina, Thierry Docoa, Stéphane Vidaa, Pascale Williamsa, Jean-Marc Brilloueta, Malcolm A. O'Neillb
Affiliations:

  • a Institut National de la Recherche Agronomique, Institut des Produits de la Vigne, Laboratoire des Polymères et des Techniques Physico-Chimiques, 2 Place Viala, F-34060 Montpellier Cedex, France

  • b Complex Carbohydrate Research Center, The University of Georgia, 220 Riverbend Road, Athens, GA 30602-4712, USA
  • Abstract: ic polysaccharide rhamnogalacturonan II (RG-II), which accounts for ~ 20% of the ethanol-precipitable polysaccharides in red wine, has been isolated from wine polysaccharides by anion-exchange chromatography. Four fractions enriched with RG-II were obtained and the RG-II then purified to homogeneity by Concanavalin A affinity and size-exclusion chromatographies. The glycosyl-residue compositions of the four RG-IIs are similar; all the RG-IIs contain the monosaccharides (apiose, , , Kdo, Dha, and aceric acid) that are diagnostic of RG-II. The glycosyl-linkages of the neutral and acidic sugars, including aceric acid, were determined simultaneously by GC-EIMS analysis of the methylated alditol acetates generated from per-O-methylated and carboxyl-reduced RG-II. Two of the RG-IIs contain boron, most likely as a borate di-ester that cross-links two molecules of RG-II together to form a dimer. The dimer contains 3′- and 2,3,3′-linked apiosyl residues whereas the monomer contains only 3′-linked apiosyl residues which suggests that the borate di-ester is located on at least one of the apiosyl residues of RG-II. Although the wine RG-IIs all have similar structures they are not identical since they differ in the length and degree of methyl-esterification of the RG-II backbone and in the presence or absence of borate di-esters. Nevertheless, these studies show that the major Structural features of wine and primary cell wall RG-II are conserved.
    Accepted: 20 May 1996
    Received: 24 July 1995
    Keywords: Rhamnogalacturonan II; Wine; Grape; Pectin; Uronic acid; Aceric acid; Homogalacturonan; Dimer; Borate di-ester
    Abbreviations: RG-II, Rhamnogalacturonan II; Kdo, acid; Dha, acid; Aceric acid, ; MALDI-TOF MS, Matrix-assisted laser desorption/ionization time of flight mass spectrometry; TMS, Per-O-trimethylsilylated methyl glycosides; CI-MS, Chemical ionization mass spectrometry; GC-EIMS, gas chromatography-electron impact mass spectrometry; ICP-AES, Inductively-coupled-plasma atomic-emission spectroscopy; HPAEC, High performance anion-exchange chromatography

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