Simultaneous detection of norovirus and rotavirus in oysters by multiplex RT–PCR
DOI: 10.1016/j.foodcont.2007.07.001
Title: Simultaneous detection of norovirus and rotavirus in oysters by multiplex RT–PCR
Journal Title: Food Control
Volume: 19
Issue: 7
Publication Date: July 2008
Start Page: 722
End Page: 726
Published online: online 6 July 2007
ISSN: 0956-7135
Author: Xiaoxia Kouab, Qingping Wua, Dapeng Wangabc, Jumei Zhanga
Affiliations:

  • a Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China

  • b Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, China

  • c Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Abstract: x reverse transcription–polymerase chain reaction (RT–PCR) was used to detect noroviruses (NVs) and rotaviruses (RVs) in artificially and naturally contaminated oysters. Clearly expected amplicons of 327 bp from NVs and 392 bp from RVs were obtained, respectively. The sensitivity was 200 pg RNA/g oyster tissue. In a total of 150 naturally contaminated oysters that were highly polluted by feces, 5 (3.33%) were rotavirus, 21 (14%) were norovirus GII and 6 (4%) were norovirus GI. The results clearly demonstrate that the multiplex RT–PCR method is a specific, sensitive and time-saving method and can be used to detect NVs and RVs in marine products before their entering the markets.
    Accepted: 2 July 2007
    Received: 21 November 2006
    Revised: 23 June 2007
    Fax: +86 20 8768 8132
    Email: dikangzhang@yahoo.com.cn

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