Technique to isolate Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) eggs
DOI: 10.1016/j.jspr.2006.11.001
Title: Technique to isolate Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) eggs
Journal Title: Journal of Stored Products Research
Volume: 43
Issue: 4
Publication Date: 2007
Start Page: 402
End Page: 403
Published online: online 8 March 2007
ISSN: 0022-474X
Author: S.G. Mummigatti, H.E. Krishnaiah
  • Food Protectants and Infestation Control Department, Central Food Technological Research Institute, Mysore 570 020, Karnataka, India
  • Abstract: Technique to isolate the eggs of Callosobruchus chinensis from the surface on which they are laid is described. An aqueous extract of their natural food, green gram, Vigna radiata, is used to coat glass beads that attract adult females for oviposition. The thin layer of extract on the glass beads allows separation of the eggs from the bead surface after a 15–20 s immersion in water. When transplanted onto fresh grain treated with glue, the separated eggs exhibit normal development and up to 97% hatching.
    Accepted: 10 November 2006
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