Superoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus)
DOI: 10.1016/j.bbrc.2003.11.022
Title: Superoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus)
Journal Title: Biochemical and Biophysical Research Communications
Volume: 312
Issue: 4
Publication Date: 26 December 2003
Start Page: 983
End Page: 988
Published online: online 19 November 2003
ISSN: 0006-291X
Author: Darren A Talbota, Nicolas Hanuiseb, Benjamin Reyb, Jean-Louis Rouanetb, Claude Duchampb, Martin D Branda
Affiliations:

  • a Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge, CB2 2XY, UK

  • b Laboratoire Physiologie Intégrative, Cellulaire et Moléculaire, CNRS-Université Lyon 1, 43 Bld 11 Novembre 1918, F-69622, Villeurbanne Cedex, France
  • Abstract: nt the partial nucleotide sequence of the avian uncoupling protein (avUCP) gene from king penguin (Aptenodytes patagonicus), showing that the protein is 88–92% identical to chicken (Gallus gallus), turkey (Meleagris gallopavo), and hummingbird (Eupetomena macroura). We show that Superoxide activates the proton conductance of mitochondria isolated from king penguin skeletal muscle. GDP abolishes the Superoxide-activated proton conductance, indicating that it is mediated via avUCP. In the absence of Superoxide there is no GDP-sensitive component of the proton conductance from penguin muscle mitochondria demonstrating that avUCP plays no role in the basal proton leak.
    Received: 6 November 2003
    Email: martin.brand@mrc

    Please Share this Paper with friends:
    Comment
    No.
    Comment Content
    User Name
    Date
    Post new Comment
    UserName
    Post to Facebook
    ×
    Search Full Text