Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells
DOI: 10.1016/S0006-291X(02)00215-2
Title: Wnt-1 promotes neuronal differentiation and inhibits gliogenesis in P19 cells
Journal Title: Biochemical and Biophysical Research Communications
Volume: 293
Issue: 1
Publication Date: 26 April 2002
Start Page: 167
End Page: 173
Published online: online 25 April 2002
ISSN: 0006-291X
Author: K Tanga, J Yanga1, X Gaoa, C Wanga, L Liua, H Kitanib, T Atsumic, N Jinga
Affiliations:

  • a Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China

  • b Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan

  • c The Laboratory of Molecular Cell Science, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
  • Abstract: Wnt-1, the vertebrate counterpart of the Drosophila wingless gene, plays an important role in the early morphogenesis of neural tissues. In this report, we have shown that overexpression of Wnt-1 can direct embryonic carcinoma P19 cells to differentiate into neuron-like cells in the absence of retinoic acid. Immunocytochemistry showed that these cells expressed neuronal markers, such as the neurofilament (NF) and microtubule-associated protein 2 (MAP2), but failed to express the glial cell marker, glial fibrillary acidic protein (GFAP). RT-PCR revealed that two basic helix-loop-helix (bHLH) genes, Mash-1 and Ngn-1, were up-regulated during the differentiation stage of Wnt-1-overexpressing P19 cells. These results suggest that the Wnt-1 gene promotes neuronal differentiation and inhibits gliogenesis during the neural differentiation of P19 cells, and that neural bHLH genes might be involved in this process.
    Received: 15 March 2002
    Keywords: Wnt-1; P19 embryonic carcinoma cell; Neural differentiation; Neuronal; Glial; bHLH genes; Mash-1; Ngn-1
    Email: njing@server.shcnc.ac.cn

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