Co-infection of methicillin-resistant Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus and extended spectrum β-lactamase producing Escherichia coli in bovine mastitis – three cases reported from India
DOI: 10.1080/01652176.2014.984365
Title: Co-infection of methicillin-resistant Staphylococcus epidermidis, methicillin-resistant Staphylococcus aureus and extended spectrum β-lactamase producing Escherichia coli in bovine mastitis – three cases reported from India
Journal Title: Veterinary Quarterly
Volume: Volume 35
Issue: Issue 1
Publication Date: January 2015
Start Page: 56
End Page: 61
Published online: 2 Dec 2014
ISSN: 0165-2176
Author: Samiran Bandyopadhyaya*, Indranil Samantab, Debaraj Bhattacharyyaa, Pramod Kumar Nandaa, Debasish Kara, Jayanta Chowdhuryc, Premanshu Dandapata, Arun Kumar Dasa, Nayan Batuld, Bimalendu Mondala, Tapan Kumar Duttae, Gunjan Dase, Bikash Chandra Dasa, Syamal Naskara, Uttam Kumar Bandyopadhyaya, Suresh Chandra Dasa & Subhasish Bandyopadhyaya
Affiliations:
a Indian Veterinary Research Institute, Eastern Regional Station, 37 Belgachia Road, Kolkata - 700 037, India
b Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Kolkata, India
c State Livestock Farm, Kalyani, India
d Department of Veterinary Medicine, West Bengal University of Animal and Fishery Sciences, Kolkata, India
e College of Veterinary Sciences, Central Agricultural University, Aizwal, India
Abstract: Emergence of antimicrobial resistance among bovine mastitis pathogens is the major cause of frequent therapeutic failure and a cause of concern for veterinary practitioners. This study describes intra-mammary infection of methicillin-resistant Staphylococcus epidermidis (MRSE), methicillin-resistant Staphylococcus aureus (MRSA) and extended spectrum β-lactamase (ESBL) producing Escherichia coli in two Holstein Friesian crossbred cows with subclinical mastitis and one non-descript cow with clinical mastitis in two different districts of West Bengal, India. In total, three MRSE, one MRSA and three ESBL producing E. coli were isolated from these cases. Both the crossbreds were detected with MRSE (HFSE1 and HFSE2) and ESBL producing E. coli (HFEC1 and HFEC2), whereas, simultaneous infection of three pathogens viz. MRSA (NDSA1), MRSE (NDSE1) and ESBL producing E. coli (NDEC1) was found in the non-descript cow. The methicillin-resistant isolates possessed mecA gene and exhibited resistance to various antibiotics such as amikacin, tetracycline and glycopeptides. The ESBL producers were positive for blaCTX-M and blaTEM genes; in addition, HFEC1 and HFEC2 were positive for blaSHV and possessed the genes for class I integron (int1), sulphonamide resistance (sul1), quinolone resistance (qnrS) and other virulence factors (papC, iucD and ESTA1). All the ESBL producers exhibited resistance to a variety of antibiotics tested including third- and fourth-generation cephalosporins and were also intermediately resistant to carbapenems. This is the first ever report on simultaneous occurrence of MRSE, MRSA and ESBL producing E. coli in bovine mastitis indicating a major concern for dairy industry and public health as well.
Accepted: 2 Nov 2014

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